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PepMix™ HIV (NEF) Ultra

PepMix™ HIV (NEF) Ultra

PepMix™ HIV (NEF) Ultra
  
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459,00 €
459,00 €

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PepMix™ HIV (NEF) Ultra

Product Code: PM-HIV-NEF

Pool of 150 15meric peptides derived from Nef protein designed to cover the high sequence diversity of the HI virus for T cell assays (e.g. ELISPOT). Protein sequences of all subtypes of HIV-1/SIVcpz based on the HIV sequence database of the Los Alamos National Lab were used. We developed a proprietary algorithm to obtain peptide combinations providing an optimal coverage of protein variability.
Amount: 1 vial containing 15 nmol (appr. 25µg) per peptide
Purity: >70% (HPLC-MS)
Delivery Format: Freeze dried in amber type glass vial
Application(s): T-cell assays, Immune monitoring, Antigen specific T-cell stimulation, T-cell expansion, Cellular immune response
Indication(s)/Topic(s): Infection, AIDS, Control
Delivery Time: 10 - 12 days

PepMixes™ HIV Ultra
The human immunodeficiency virus (HIV) is the causative agent of the acquired immunodeficiency syndrome (AIDS), a disease, by which over 25 million people were killed in the past three decades. The major difficulty with HIV is the extremely high mutation rate of the viral genome leading to an enormous diversity of the HIV proteome, and allowing the virus to escape the human immune response. Conventional consensus based peptide pools address this problem only very poorly. Therefore, when designing the new HIV-1 ENV, GAG, NEF and POL Ultra PepMix™ Peptide Pools, we focused primarily on coverage of the tremendous sequence diversity of HIV. Protein sequences of all subtypes of HIV-1/SIVcpz from the Los Alamos National Lab were considered. We developed a proprietary algorithm to obtain peptide combinations providing optimal coverage of the protein variability. Broad clade coverage provided by these new peptide pools enables stimulation and monitoring of immune responses in HIV patients independent of geographical origin.

Benefits of PepMix™
- Equivalent or better stimulation of CD4+ and CD8+ T-cell responses compared to whole protein antigens
- Simultaneous detection of CD4+ and CD8+ responses in a single sample
- Improved responses in stored blood and PBM cells compared to whole protein antigens
- More reliable as control than phytohemagglutinin (PHA) and concanavalin A (ConA)
- High-batch-to-batch reproducibility
- Reliable chemical and biochemical quality control and quality assurance for peptide synthesis and peptide pool generation
- Prolonged shelf stability when stored freeze dried

PepMix™ HIV (NEF) Ultra

References:
Read References with PepMix™ Peptide Pools - Infectious Diseases

Application Notes
"Developing Multi-HIV Antigen Specific T-Cells as a Component of a Cure Strategy"
Lam et al. (2015) Full text
"Peptide-stimulated Expansion of Virus-specific T cells For Preventative Treatment After Allogeneic Stem Cell Transplantation"
Gary et al. (2015) Full text

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X

Testimonials
"To establish a novel role for myeloid derived suppressor cells (Pallett et al, Nat. Med. 2015) in chronic viral infection, we utilised the PepMix CEF Pool (extended) as well as a custom synthesized PepMix spanning the core region of HBV genotype D . Whereas, the CEF peptide pool consists of 32 peptides, each corresponding to a defined HLA class I-restricted T-cell epitope from Cytomegalo, Epstein-Barr, and Influenza virus, the latter custom PepMix included 15meric peptides overlapping by 10 amino acids. Specifically this composition enabled us to monitor both the antiviral CD8+ and CD4+ T cell responses in chronic HBV infection and the non-antigen specific T cells that are known to mediate immunopathology in the liver. Our entire experience with JPT, from ordering/delivery to use in the lab was excellent. Not only were the reagents able to perform reliably and consistently in vitro from batch to batch, the customer and technical support provided was continually available and efficient when needed. JPT will remain our "go-to" company for purchasing peptides."
Dr. Laura J Pallett, Infection and Immunity, University College London, UK

PepMix™ HIV (NEF) Ultra

 
Code
Product Name
 
Price
Buy
PM-HIV-ENV
PepMix™ HIV (ENV) Ultra
Pool of 150 15meric peptides derived from Envelope glycoprotein gp160 designed to cover the high sequence diversity of the HI virus for T cell assays
add to cart
PM-HIV-POL
PepMix™ HIV (POL) Ultra
Pool of 150 15meric peptides derived from Pol Polyprotein designed to cover the high sequence diversity of the HI virus for T cell assays
add to cart
RT-HD-HIV
RepliTope™ Antigen Collection HIV Ultra
Microarray combining an overlapping peptide scan through the full length sequence of HIV gag p17 and p24, tat, nef and env plus the remaining immunogenic regions of the HIV prot...
add to cart

Description

Product Code: PM-HIV-NEF

Pool of 150 15meric peptides derived from Nef protein designed to cover the high sequence diversity of the HI virus for T cell assays (e.g. ELISPOT). Protein sequences of all subtypes of HIV-1/SIVcpz based on the HIV sequence database of the Los Alamos National Lab were used. We developed a proprietary algorithm to obtain peptide combinations providing an optimal coverage of protein variability.
Amount: 1 vial containing 15 nmol (appr. 25µg) per peptide
Purity: >70% (HPLC-MS)
Delivery Format: Freeze dried in amber type glass vial
Application(s): T-cell assays, Immune monitoring, Antigen specific T-cell stimulation, T-cell expansion, Cellular immune response
Indication(s)/Topic(s): Infection, AIDS, Control
Delivery Time: 10 - 12 days

PepMixes™ HIV Ultra
The human immunodeficiency virus (HIV) is the causative agent of the acquired immunodeficiency syndrome (AIDS), a disease, by which over 25 million people were killed in the past three decades. The major difficulty with HIV is the extremely high mutation rate of the viral genome leading to an enormous diversity of the HIV proteome, and allowing the virus to escape the human immune response. Conventional consensus based peptide pools address this problem only very poorly. Therefore, when designing the new HIV-1 ENV, GAG, NEF and POL Ultra PepMix™ Peptide Pools, we focused primarily on coverage of the tremendous sequence diversity of HIV. Protein sequences of all subtypes of HIV-1/SIVcpz from the Los Alamos National Lab were considered. We developed a proprietary algorithm to obtain peptide combinations providing optimal coverage of the protein variability. Broad clade coverage provided by these new peptide pools enables stimulation and monitoring of immune responses in HIV patients independent of geographical origin.

Benefits of PepMix™
- Equivalent or better stimulation of CD4+ and CD8+ T-cell responses compared to whole protein antigens
- Simultaneous detection of CD4+ and CD8+ responses in a single sample
- Improved responses in stored blood and PBM cells compared to whole protein antigens
- More reliable as control than phytohemagglutinin (PHA) and concanavalin A (ConA)
- High-batch-to-batch reproducibility
- Reliable chemical and biochemical quality control and quality assurance for peptide synthesis and peptide pool generation
- Prolonged shelf stability when stored freeze dried

References

References:
Read References with PepMix™ Peptide Pools - Infectious Diseases

Application Notes
"Developing Multi-HIV Antigen Specific T-Cells as a Component of a Cure Strategy"
Lam et al. (2015) Full text
"Peptide-stimulated Expansion of Virus-specific T cells For Preventative Treatment After Allogeneic Stem Cell Transplantation"
Gary et al. (2015) Full text

_x005F

X

Testimonials
"To establish a novel role for myeloid derived suppressor cells (Pallett et al, Nat. Med. 2015) in chronic viral infection, we utilised the PepMix CEF Pool (extended) as well as a custom synthesized PepMix spanning the core region of HBV genotype D . Whereas, the CEF peptide pool consists of 32 peptides, each corresponding to a defined HLA class I-restricted T-cell epitope from Cytomegalo, Epstein-Barr, and Influenza virus, the latter custom PepMix included 15meric peptides overlapping by 10 amino acids. Specifically this composition enabled us to monitor both the antiviral CD8+ and CD4+ T cell responses in chronic HBV infection and the non-antigen specific T cells that are known to mediate immunopathology in the liver. Our entire experience with JPT, from ordering/delivery to use in the lab was excellent. Not only were the reagents able to perform reliably and consistently in vitro from batch to batch, the customer and technical support provided was continually available and efficient when needed. JPT will remain our "go-to" company for purchasing peptides."
Dr. Laura J Pallett, Infection and Immunity, University College London, UK

Documentation

Properties

Related Products

PM-HIV-ENV
PepMix™ HIV (ENV) Ultra
Pool of 150 15meric peptides derived from Envelope glycoprotein gp160 designed to cover the high sequence diversity of the HI virus for T cell assays
add to cart
PM-HIV-POL
PepMix™ HIV (POL) Ultra
Pool of 150 15meric peptides derived from Pol Polyprotein designed to cover the high sequence diversity of the HI virus for T cell assays
add to cart
RT-HD-HIV
RepliTope™ Antigen Collection HIV Ultra
Microarray combining an overlapping peptide scan through the full length sequence of HIV gag p17 and p24, tat, nef and env plus the remaining immunogenic regions of the HIV prot...
add to cart

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JPT Peptide Technologies GmbH
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Technical Support:
T: +49-30-6392-7878
X: +49-30-6392-7888
e-mail: peptide@jpt.com
USA & Canada:
T: 1.888.JPT.COM0 (1.888.578.2660)
X: 1.888.JPT.COM6 (1.888.578.2666)
e-mail: peptide@jpt.com