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PepMix™ SARS-CoV-2 (ORF10)

PepMix™ SARS-CoV-2 (ORF10)
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PepMix™ SARS-CoV-2 (ORF10)

Product Code: PM-WCPV-ORF10

Pool of 7 peptides derived from a peptide scan (15mers with 11 aa overlap) through ORF10 Protein (UniProt: A0A663DJA2) of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g. ELISPOT).
Amount: 1 vial containing 15 nmol (appr. 25µg) per peptide
Purity: Please select between ">70%" (for each peptide) or "crude with guarantee" (major peak by LC-MS is guaranteed to be peptide of interest for each peptide & cytotoxicity-tested)
Delivery Format: Freeze dried in amber type glass vial
Application(s): T-cell assays, Immune monitoring, Antigen specific T-cell stimulation, T-cell expansion, Cellular immune response
Indication(s)/Topic(s): Covid-19, Infection, Respiratory infection
Delivery Time: 2-5 days
Complete Product Codes: PM-WCPV-ORF10-1 (crude), PM-WCPV-ORF10-2 (>70%)

PepMix™ Peptide Pools
The use of complete protein-spanning mixtures of overlapping peptides (PepMix) is extremely efficient for immunostimulation of T-lymphocytes and diagnostic applications. In order to establish reliable and validated T-cell assays such as ELISPOT, appropriate positive and negative controls are essential. Selection of such controls helps to confirm proper functionality of the assay as well as viability of the cells used. In addition, high quality controls are most important to establish standardized assay protocols in clinical setups. Compared to commonly used controls like phytohemagglutinin (PHA) and concanavalin A (ConA) that stimulate T-cells as well as other cell types or full length antigens, synthetic peptide pools offer the advantage of a high batch-to-batch reproducibility, applicability of reliable chemical and biochemical QC/QA measures and shelf stability when stored as freeze dried aliquots.

Benefits of PepMix™ Peptide Pools
- Equivalent or better stimulation of CD4+ and CD8+ antigen-specific T-cell responses compared to whole protein antigens
- Simultaneous detection of CD4+ and CD8+ responses in a single sample
- Improved responses in stored blood and PBM cells compared to whole protein antigens

PepMix™ SARS-CoV-2 (ORF10)

References:
Read References with PepMix™ Peptide Pools - Infectious Diseases

Application Notes
"Developing Multi-HIV Antigen Specific T-Cells as a Component of a Cure Strategy"
Lam et al. (2015) Full text
"Peptide-stimulated Expansion of Virus-specific T cells For Preventative Treatment After Allogeneic Stem Cell Transplantation"
Gary et al. (2015) Full text

Testimonials
"To establish a novel role for myeloid derived suppressor cells (Pallett et al, Nat. Med. 2015) in chronic viral infection, we utilised the PepMix CEF Pool (extended) as well as a custom synthesized PepMix spanning the core region of HBV genotype D . Whereas, the CEF peptide pool consists of 32 peptides, each corresponding to a defined HLA class I-restricted T-cell epitope from Cytomegalo, Epstein-Barr, and Influenza virus, the latter custom PepMix included 15meric peptides overlapping by 10 amino acids. Specifically this composition enabled us to monitor both the antiviral CD8+ and CD4+ T cell responses in chronic HBV infection and the non-antigen specific T cells that are known to mediate immunopathology in the liver. Our entire experience with JPT, from ordering/delivery to use in the lab was excellent. Not only were the reagents able to perform reliably and consistently in vitro from batch to batch, the customer and technical support provided was continually available and efficient when needed. JPT will remain our "go-to" company for purchasing peptides."
Dr. Laura J Pallett, Infection and Immunity, University College London, UK

PepMix™ SARS-CoV-2 (ORF10)

 
Code
Product Name
 
Price
Buy
PM-CEFX
CEFX Ultra SuperStim Pool
Positive Control Pool of 176 known peptide epitopes for a broad range of HLA sub-types and different infectious agents for T-cell stimulation of populations with a diverse ethni...
add to cart
PM-WCPV-S
PepMix™ SARS-CoV-2 (Spike Glycoprotein)
Pool of 315 peptides (delivered in two subpools of 158 & 157 peptides) derived from a peptide scan through the entire Spike glycoprotein of SARS-CoV-2 (Severe Acute Respiratory ...
add to cart
PM-WCPV-NS7B
PepMix™ SARS-CoV-2 (NS7B)
Pool of 8 peptides derived from a peptide scan through Non-structural Protein 7b of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g. ...
add to cart
PM-WCPV-ORF9B
PepMix™ SARS-CoV-2 (ORF9B)
Pool of 22 peptides derived from a peptide scan through Protein 9b of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g. ELISPOT)
add to cart
PM-WCPV-Y14
PepMix™ SARS-CoV-2 (Y14)
Pool of 16 peptides derived from a peptide scan through Uncharacterized protein 14 of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g...
add to cart
PM-229E-S-1
PepMix™ HCoV-229E (Spike Glycoprotein)
Pool of 291 peptides (delivered in two subpools of 146 & 145 peptides) derived from a peptide scan through the entire Spike glycoprotein of Human coronavirus 229E (HCoV-229E) fo...
add to cart
PM-OC43-S-1
PepMix™ HCoV-OC43 (Spike Glycoprotein)
Pool of 336 peptides (delivered in two subpools of 168 peptides each) derived from a peptide scan through the entire Spike glycoprotein of Human coronavirus OC43 (HCoV-OC43) for...
add to cart
PM-WCPV-ORF10
PepMix™ SARS-CoV-2 (ORF10)
Pool of 7 peptides derived from a peptide scan through ORF10 Protein of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g. ELISPOT)
add to cart
PM-WCPV-NCAP
PepMix™ SARS-CoV-2 (NCAP)
Pool of 102 peptides derived from a peptide scan through Nucleoprotein of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g. ELISPOT)
add to cart
PM-WCPV-NS8
PepMix™ SARS-CoV-2 (NS8)
Pool of 28 peptides derived from a peptide scan through Non-structural protein 8 of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g. ...
add to cart
PM-WCPV-NS7A
PepMix™ SARS-CoV-2 (NS7a)
Pool of 28 peptides derived from a peptide scan through Non-structural protein 7A of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g....
add to cart
PM-WCPV-NS6
PepMix™ SARS-CoV-2 (NS6)
Pool of 13 peptides derived from a peptide scan through Non-Structural protein 6 of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g. ...
add to cart
PM-WCPV-VME
PepMix™ SARS-CoV-2 (VME1)
Pool of 53 peptides derived from a peptide scan through Membrane protein of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g. ELISPOT)
add to cart
PM-WCPV-VEMP
PepMix™ SARS-CoV-2 (VEMP)
Pool of 16 peptides derived from a peptide scan through Envelope small membrane protein of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays...
add to cart
PM-WCPV-AP3A
PepMix™ SARS-CoV-2 (AP3A)
Pool of 66 peptides derived from a peptide scan through Protein 3a of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g. ELISPOT)
add to cart
PM-WCPV-Nsp12-1
PepMix™ SARS-CoV-2 (Nsp12)
Pool of 231 peptides derived from a peptide scan through RNA-directed RNA Polymerase of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e...
add to cart
PM-WCPV-Nsp5-1
PepMix™ SARS-CoV-2 (Nsp5)
Pool of 74 peptides derived from a peptide scan through RNA-directed 3c-like Proteinase of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays...
add to cart
PM-NL63-S-1
PepMix™ HCoV-NL63 (Spike Glycoprotein)
Pool of 337 peptides (delivered in two subpools of 169 & 168 peptides) derived from a peptide scan through Spike glycoprotein of Human coronavirus NL63 (HCoV-NL63) for T cell as...
add to cart
PM-HKU1-S-1
PepMix™ HCoV- HKU1 (Spike Glycoprotein)
Pool of 327 peptides (delivered in two subpools of 169 & 168 peptides) derived from a peptide scan through Spike glycoprotein of Human coronavirus HKU1 (HCoV-HKU1) (isolate N1) ...
add to cart
PM-WCPV-S-RBD-1
PepMix™ SARS-CoV-2 (S-RBD)
Pool of 53 peptides derived from a peptide scan through Receptor Binding Domain of Spike Glycoprotein of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for...
add to cart
RT-HD-PanFl
RepliTope™ Antigen Collection Pan-Flavivirus Ultra (Zika)
Peptide microarray displaying 6253 peptides derived from selected proteins of ZIKA virus, Dengue virus, West Nile virus, Yellow fever virus and Saint Louis encephalitis virus
add to cart
EMPS-WCPV-NCAP-1
Epitope Mapping Peptide Set SARS-CoV-2 (NCAP)
Set of 21 Matrix Pools and 102 individual overlapping peptides spanning the whole Nucleoprotein of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for epito...
add to cart
EMPS-WCPV-S-1
Epitope Mapping Peptide Set SARS-CoV-2 (Spike Glycoprotein)
Set of 52 Matrix Pools and 315 individual overlapping peptides spanning the whole Spike glycoprotein of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for ...
add to cart

Description

Product Code: PM-WCPV-ORF10

Pool of 7 peptides derived from a peptide scan (15mers with 11 aa overlap) through ORF10 Protein (UniProt: A0A663DJA2) of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g. ELISPOT).
Amount: 1 vial containing 15 nmol (appr. 25µg) per peptide
Purity: Please select between ">70%" (for each peptide) or "crude with guarantee" (major peak by LC-MS is guaranteed to be peptide of interest for each peptide & cytotoxicity-tested)
Delivery Format: Freeze dried in amber type glass vial
Application(s): T-cell assays, Immune monitoring, Antigen specific T-cell stimulation, T-cell expansion, Cellular immune response
Indication(s)/Topic(s): Covid-19, Infection, Respiratory infection
Delivery Time: 2-5 days
Complete Product Codes: PM-WCPV-ORF10-1 (crude), PM-WCPV-ORF10-2 (>70%)

PepMix™ Peptide Pools
The use of complete protein-spanning mixtures of overlapping peptides (PepMix) is extremely efficient for immunostimulation of T-lymphocytes and diagnostic applications. In order to establish reliable and validated T-cell assays such as ELISPOT, appropriate positive and negative controls are essential. Selection of such controls helps to confirm proper functionality of the assay as well as viability of the cells used. In addition, high quality controls are most important to establish standardized assay protocols in clinical setups. Compared to commonly used controls like phytohemagglutinin (PHA) and concanavalin A (ConA) that stimulate T-cells as well as other cell types or full length antigens, synthetic peptide pools offer the advantage of a high batch-to-batch reproducibility, applicability of reliable chemical and biochemical QC/QA measures and shelf stability when stored as freeze dried aliquots.

Benefits of PepMix™ Peptide Pools
- Equivalent or better stimulation of CD4+ and CD8+ antigen-specific T-cell responses compared to whole protein antigens
- Simultaneous detection of CD4+ and CD8+ responses in a single sample
- Improved responses in stored blood and PBM cells compared to whole protein antigens

References

References:
Read References with PepMix™ Peptide Pools - Infectious Diseases

Application Notes
"Developing Multi-HIV Antigen Specific T-Cells as a Component of a Cure Strategy"
Lam et al. (2015) Full text
"Peptide-stimulated Expansion of Virus-specific T cells For Preventative Treatment After Allogeneic Stem Cell Transplantation"
Gary et al. (2015) Full text

Testimonials
"To establish a novel role for myeloid derived suppressor cells (Pallett et al, Nat. Med. 2015) in chronic viral infection, we utilised the PepMix CEF Pool (extended) as well as a custom synthesized PepMix spanning the core region of HBV genotype D . Whereas, the CEF peptide pool consists of 32 peptides, each corresponding to a defined HLA class I-restricted T-cell epitope from Cytomegalo, Epstein-Barr, and Influenza virus, the latter custom PepMix included 15meric peptides overlapping by 10 amino acids. Specifically this composition enabled us to monitor both the antiviral CD8+ and CD4+ T cell responses in chronic HBV infection and the non-antigen specific T cells that are known to mediate immunopathology in the liver. Our entire experience with JPT, from ordering/delivery to use in the lab was excellent. Not only were the reagents able to perform reliably and consistently in vitro from batch to batch, the customer and technical support provided was continually available and efficient when needed. JPT will remain our "go-to" company for purchasing peptides."
Dr. Laura J Pallett, Infection and Immunity, University College London, UK

Documentation

Properties

Related Products

PM-CEFX
CEFX Ultra SuperStim Pool
Positive Control Pool of 176 known peptide epitopes for a broad range of HLA sub-types and different infectious agents for T-cell stimulation of populations with a diverse ethni...
add to cart
PM-WCPV-S
PepMix™ SARS-CoV-2 (Spike Glycoprotein)
Pool of 315 peptides (delivered in two subpools of 158 & 157 peptides) derived from a peptide scan through the entire Spike glycoprotein of SARS-CoV-2 (Severe Acute Respiratory ...
add to cart
PM-WCPV-NS7B
PepMix™ SARS-CoV-2 (NS7B)
Pool of 8 peptides derived from a peptide scan through Non-structural Protein 7b of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g. ...
add to cart
PM-WCPV-ORF9B
PepMix™ SARS-CoV-2 (ORF9B)
Pool of 22 peptides derived from a peptide scan through Protein 9b of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g. ELISPOT)
add to cart
PM-WCPV-Y14
PepMix™ SARS-CoV-2 (Y14)
Pool of 16 peptides derived from a peptide scan through Uncharacterized protein 14 of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g...
add to cart
PM-229E-S-1
PepMix™ HCoV-229E (Spike Glycoprotein)
Pool of 291 peptides (delivered in two subpools of 146 & 145 peptides) derived from a peptide scan through the entire Spike glycoprotein of Human coronavirus 229E (HCoV-229E) fo...
add to cart
PM-OC43-S-1
PepMix™ HCoV-OC43 (Spike Glycoprotein)
Pool of 336 peptides (delivered in two subpools of 168 peptides each) derived from a peptide scan through the entire Spike glycoprotein of Human coronavirus OC43 (HCoV-OC43) for...
add to cart
PM-WCPV-ORF10
PepMix™ SARS-CoV-2 (ORF10)
Pool of 7 peptides derived from a peptide scan through ORF10 Protein of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g. ELISPOT)
add to cart
PM-WCPV-NCAP
PepMix™ SARS-CoV-2 (NCAP)
Pool of 102 peptides derived from a peptide scan through Nucleoprotein of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g. ELISPOT)
add to cart
PM-WCPV-NS8
PepMix™ SARS-CoV-2 (NS8)
Pool of 28 peptides derived from a peptide scan through Non-structural protein 8 of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g. ...
add to cart
PM-WCPV-NS7A
PepMix™ SARS-CoV-2 (NS7a)
Pool of 28 peptides derived from a peptide scan through Non-structural protein 7A of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g....
add to cart
PM-WCPV-NS6
PepMix™ SARS-CoV-2 (NS6)
Pool of 13 peptides derived from a peptide scan through Non-Structural protein 6 of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g. ...
add to cart
PM-WCPV-VME
PepMix™ SARS-CoV-2 (VME1)
Pool of 53 peptides derived from a peptide scan through Membrane protein of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g. ELISPOT)
add to cart
PM-WCPV-VEMP
PepMix™ SARS-CoV-2 (VEMP)
Pool of 16 peptides derived from a peptide scan through Envelope small membrane protein of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays...
add to cart
PM-WCPV-AP3A
PepMix™ SARS-CoV-2 (AP3A)
Pool of 66 peptides derived from a peptide scan through Protein 3a of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e.g. ELISPOT)
add to cart
PM-WCPV-Nsp12-1
PepMix™ SARS-CoV-2 (Nsp12)
Pool of 231 peptides derived from a peptide scan through RNA-directed RNA Polymerase of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays (e...
add to cart
PM-WCPV-Nsp5-1
PepMix™ SARS-CoV-2 (Nsp5)
Pool of 74 peptides derived from a peptide scan through RNA-directed 3c-like Proteinase of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for T cell assays...
add to cart
PM-NL63-S-1
PepMix™ HCoV-NL63 (Spike Glycoprotein)
Pool of 337 peptides (delivered in two subpools of 169 & 168 peptides) derived from a peptide scan through Spike glycoprotein of Human coronavirus NL63 (HCoV-NL63) for T cell as...
add to cart
PM-HKU1-S-1
PepMix™ HCoV- HKU1 (Spike Glycoprotein)
Pool of 327 peptides (delivered in two subpools of 169 & 168 peptides) derived from a peptide scan through Spike glycoprotein of Human coronavirus HKU1 (HCoV-HKU1) (isolate N1) ...
add to cart
PM-WCPV-S-RBD-1
PepMix™ SARS-CoV-2 (S-RBD)
Pool of 53 peptides derived from a peptide scan through Receptor Binding Domain of Spike Glycoprotein of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for...
add to cart
RT-HD-PanFl
RepliTope™ Antigen Collection Pan-Flavivirus Ultra (Zika)
Peptide microarray displaying 6253 peptides derived from selected proteins of ZIKA virus, Dengue virus, West Nile virus, Yellow fever virus and Saint Louis encephalitis virus
add to cart
EMPS-WCPV-NCAP-1
Epitope Mapping Peptide Set SARS-CoV-2 (NCAP)
Set of 21 Matrix Pools and 102 individual overlapping peptides spanning the whole Nucleoprotein of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for epito...
add to cart
EMPS-WCPV-S-1
Epitope Mapping Peptide Set SARS-CoV-2 (Spike Glycoprotein)
Set of 52 Matrix Pools and 315 individual overlapping peptides spanning the whole Spike glycoprotein of SARS-CoV-2 (Severe Acute Respiratory Syndrome-related coronavirus 2) for ...
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News

SARS COV-2 Mass Spec Reference Kit Released!:

SARS-CoV-2 SpikeMix  for Mass Spectrometry covers spike, NCAP, VME-1 & ORF9B
(July 2020)
Read more

New! SARS-CoV-2 Epitope Mapping Peptide Sets

Save time and samples and check our new SARS-CoV-2 Epitope Mapping Peptide Sets (EMPS).
(Apr 2020)
Read more


> Read more news!

Quality Assurance

All production is performed according to ISO 9001:2015 standards.

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Contact us:

JPT Peptide Technologies GmbH
Volmerstrasse 5
12489 Berlin, Germany
Technical Support:
T: +49-30-6392-7878
X: +49-30-6392-7888
e-mail: peptide@jpt.com
USA & Canada:
T: 1.888.JPT.COM0 (1.888.578.2660)
X: 1.888.JPT.COM6 (1.888.578.2666)
e-mail: peptide@jpt.com