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PepMix™ Influenza A (NP (H3N2))

PepMix™ Influenza A (NP (H3N2))

PepMix™ Influenza A (NP (H3N2))
  
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Delivery in 2-5 days
331,00 €
331,00 €

Excluding tax
prices do not include tax. For shipment within Germany 19% MwSt will be included at checkout and in the invoice. For shipment within EU-countries you will need to provide the valid VAT number. International customers should note that all duties, import fees, and taxes are to be paid by the customer
and
shipping fees
Combine this product with our corresponding Influenza NP Products: PepMix™ Peptide Pools and Antigen Peptides.

PepMix™ Influenza A (NP (H3N2))

Product Code: PM-INFA_NP

Pool of122 peptides derived from a peptide scan (15mers with 11 aa overlap) through Nucleocapsid protein (Swiss-Prot ID: O91743) of Influenza A virus (strain A/Kitakyushu/159/1993 H3N2) for T cell assays.
Amount: 1 vial containing 15 nmol (appr. 25µg) per peptide
Purity: >70% (HPLC-MS)
Delivery Format: Freeze dried in amber type glass vial
Application(s): T-cell assays, Immune monitoring, Antigen specific T-cell stimulation, T-cell expansion, Cellular immune response
Indication(s)/Topic(s): Infection, Influenza, Seasonal influenza, Respiratory infection
Delivery Time: 4-5 days

PepMix™ Peptide Pools
The use of complete protein-spanning mixtures of overlapping peptides (PepMix) is extremely efficient for immunostimulation of T-lymphocytes and diagnostic applications. In order to establish reliable and validated T-cell assays such as ELISPOT, appropriate positive and negative controls are essential. Selection of such controls helps to confirm proper functionality of the assay as well as viability of the cells used. In addition, high quality controls are most important to establish standardized assay protocols in clinical setups. Compared to commonly used controls like phytohemagglutinin (PHA) and concanavalin A (ConA) that stimulate T-cells as well as other cell types or full length antigens, synthetic peptide pools offer the advantage of a high batch-to-batch reproducibility, applicability of reliable chemical and biochemical QC/QA measures and shelf stability when stored as freeze dried aliquots.

Benefits of PepMix™
- Equivalent or better stimulation of CD4+ and CD8+ T-cell responses compared to whole protein antigens
- Simultaneous detection of CD4+ and CD8+ responses in a single sample
- Improved responses in stored blood and PBM cells compared to whole protein antigens
- More reliable as control than phytohemagglutinin (PHA) and concanavalin A (ConA)
- High-batch-to-batch reproducibility
- Reliable chemical and biochemical quality control and quality assurance for peptide synthesis and peptide pool generation
- Prolonged shelf stability when stored freeze dried

PepMix™ Influenza A (NP (H3N2))

References:
Read References with PepMix™ Peptide Pools - Infectious Diseases

Application Notes
"Developing Multi-HIV Antigen Specific T-Cells as a Component of a Cure Strategy"
Lam et al. (2015) Full text
"Peptide-stimulated Expansion of Virus-specific T cells For Preventative Treatment After Allogeneic Stem Cell Transplantation"
Gary et al. (2015) Full text

Testimonials
"To establish a novel role for myeloid derived suppressor cells (Pallett et al, Nat. Med. 2015) in chronic viral infection, we utilised the PepMix CEF Pool (extended) as well as a custom synthesized PepMix spanning the core region of HBV genotype D . Whereas, the CEF peptide pool consists of 32 peptides, each corresponding to a defined HLA class I-restricted T-cell epitope from Cytomegalo, Epstein-Barr, and Influenza virus, the latter custom PepMix included 15meric peptides overlapping by 10 amino acids. Specifically this composition enabled us to monitor both the antiviral CD8+ and CD4+ T cell responses in chronic HBV infection and the non-antigen specific T cells that are known to mediate immunopathology in the liver. Our entire experience with JPT, from ordering/delivery to use in the lab was excellent. Not only were the reagents able to perform reliably and consistently in vitro from batch to batch, the customer and technical support provided was continually available and efficient when needed. JPT will remain our "go-to" company for purchasing peptides."
Dr. Laura J Pallett, Infection and Immunity, University College London, UK

PepMix™ Influenza A (NP (H3N2))

 
Code
Product Name
 
Price
Buy
PM-INFA-HABris
PepMix™ Influenza A (HA /Brisbane (H1N1))
Pool of 139 peptides derived from a peptide scan through Hemagglutinin of Influenza A virus (A/Brisbane/59/2007(H1N1)) for T cell assays
add to cart
PM-INFA-HACal
PepMix™ Influenza A (HA /California (H1N1))
Pool of 139 peptides derived from a peptide scan through Hemagglutinin of Influenza A virus (A/California/07/2009(H1N1)) for T cell assays
add to cart
PM-INFA_MP1
PepMix™ Influenza A (MP1 (H3N2))
Pool of 61 peptides derived from a peptide scan through Matrix protein 1 of Influenza A virus (strain A/Aichi/2/1968 H3N2) for T cell assays
add to cart
PM-INFA-MP1-H1N1
PepMix™ Influenza A (MP1 /California (H1N1))
Pool of 61 peptides derived from a peptide scan through Matrix protein 1 of Influenza A virus (A/California/08/2009(H1N1)) for T cell assays
add to cart
PM-INFA_MP2
PepMix™ Influenza A (MP2 /California (H1N1))
Pool of 22 peptides derived from a peptide scan through Matrix protein 2 of Influenza A virus (A/California/08/2009(H1N1)) for T cell assays
add to cart
PM-INFA_NA
PepMix™ Influenza A (Neuroamidase /California (H1N1)
Pool of 115 peptides derived from a peptide scan through Neuraminidase of Influenza A virus (A/California/08/2009(H1N1)) for T cell assays
add to cart
PM-INFA_NP
PepMix™ Influenza A (NP (H3N2))
Pool of 122 peptides derived from a peptide scan through Nucleocapsid protein of Influenza A virus (strain A/Kitakyushu/159/1993 H3N2) for T cell assays
add to cart
RT-INFA-HA
RepliTope™ Influenza A (HA /Brisbane (H1N1))
Peptide microarray displaying 139 peptides derived from a peptide scan through Hemagglutinin of Influenza A virus (A/Brisbane/59/2007(H1N1))
add to cart
RT-INFA-HACal
RepliTope™ Influenza A (HA /California (H1N1))
Peptide microarray displaying 140 peptides derived from a peptide scan through Hemagglutinin of Influenza A virus (A/California/08/2009(H1N1))
add to cart
RT-INFA_MP1
RepliTope™ Influenza A (MP1 (H3N2))
Peptide microarray displaying 61 peptides derived from a peptide scan through Matrix protein 1 of Influenza A virus (strain A/Aichi/2/1968 (H3N2))
add to cart
RT-INFA-MP1-H1N1
RepliTope™ Influenza A (MP1 /California (H1N1))
Peptide microarray displaying 61 peptides derived from a peptide scan through Matrix protein 1 of Influenza A virus (A/California/08/2009(H1N1))
add to cart
RT-INFA_MP2
RepliTope™ Influenza A (MP2 /California (H1N1))
Peptide microarray displaying 22 peptides derived from a peptide scan through Matrix protein 2 of Influenza A virus (A/California/08/2009(H1N1))
add to cart
RT-INFA_NA
RepliTope™ Influenza A (Neuroamidase /California (H1N1)
Peptide microarray displaying 115 peptides derived from a peptide scan through Neuraminidase of Influenza A virus (A/California/08/2009(H1N1))
add to cart

Description

Product Code: PM-INFA_NP

Pool of122 peptides derived from a peptide scan (15mers with 11 aa overlap) through Nucleocapsid protein (Swiss-Prot ID: O91743) of Influenza A virus (strain A/Kitakyushu/159/1993 H3N2) for T cell assays.
Amount: 1 vial containing 15 nmol (appr. 25µg) per peptide
Purity: >70% (HPLC-MS)
Delivery Format: Freeze dried in amber type glass vial
Application(s): T-cell assays, Immune monitoring, Antigen specific T-cell stimulation, T-cell expansion, Cellular immune response
Indication(s)/Topic(s): Infection, Influenza, Seasonal influenza, Respiratory infection
Delivery Time: 4-5 days

PepMix™ Peptide Pools
The use of complete protein-spanning mixtures of overlapping peptides (PepMix) is extremely efficient for immunostimulation of T-lymphocytes and diagnostic applications. In order to establish reliable and validated T-cell assays such as ELISPOT, appropriate positive and negative controls are essential. Selection of such controls helps to confirm proper functionality of the assay as well as viability of the cells used. In addition, high quality controls are most important to establish standardized assay protocols in clinical setups. Compared to commonly used controls like phytohemagglutinin (PHA) and concanavalin A (ConA) that stimulate T-cells as well as other cell types or full length antigens, synthetic peptide pools offer the advantage of a high batch-to-batch reproducibility, applicability of reliable chemical and biochemical QC/QA measures and shelf stability when stored as freeze dried aliquots.

Benefits of PepMix™
- Equivalent or better stimulation of CD4+ and CD8+ T-cell responses compared to whole protein antigens
- Simultaneous detection of CD4+ and CD8+ responses in a single sample
- Improved responses in stored blood and PBM cells compared to whole protein antigens
- More reliable as control than phytohemagglutinin (PHA) and concanavalin A (ConA)
- High-batch-to-batch reproducibility
- Reliable chemical and biochemical quality control and quality assurance for peptide synthesis and peptide pool generation
- Prolonged shelf stability when stored freeze dried

References

References:
Read References with PepMix™ Peptide Pools - Infectious Diseases

Application Notes
"Developing Multi-HIV Antigen Specific T-Cells as a Component of a Cure Strategy"
Lam et al. (2015) Full text
"Peptide-stimulated Expansion of Virus-specific T cells For Preventative Treatment After Allogeneic Stem Cell Transplantation"
Gary et al. (2015) Full text

Testimonials
"To establish a novel role for myeloid derived suppressor cells (Pallett et al, Nat. Med. 2015) in chronic viral infection, we utilised the PepMix CEF Pool (extended) as well as a custom synthesized PepMix spanning the core region of HBV genotype D . Whereas, the CEF peptide pool consists of 32 peptides, each corresponding to a defined HLA class I-restricted T-cell epitope from Cytomegalo, Epstein-Barr, and Influenza virus, the latter custom PepMix included 15meric peptides overlapping by 10 amino acids. Specifically this composition enabled us to monitor both the antiviral CD8+ and CD4+ T cell responses in chronic HBV infection and the non-antigen specific T cells that are known to mediate immunopathology in the liver. Our entire experience with JPT, from ordering/delivery to use in the lab was excellent. Not only were the reagents able to perform reliably and consistently in vitro from batch to batch, the customer and technical support provided was continually available and efficient when needed. JPT will remain our "go-to" company for purchasing peptides."
Dr. Laura J Pallett, Infection and Immunity, University College London, UK

Documentation

Properties

Related Products

PM-INFA-HABris
PepMix™ Influenza A (HA /Brisbane (H1N1))
Pool of 139 peptides derived from a peptide scan through Hemagglutinin of Influenza A virus (A/Brisbane/59/2007(H1N1)) for T cell assays
add to cart
PM-INFA-HACal
PepMix™ Influenza A (HA /California (H1N1))
Pool of 139 peptides derived from a peptide scan through Hemagglutinin of Influenza A virus (A/California/07/2009(H1N1)) for T cell assays
add to cart
PM-INFA_MP1
PepMix™ Influenza A (MP1 (H3N2))
Pool of 61 peptides derived from a peptide scan through Matrix protein 1 of Influenza A virus (strain A/Aichi/2/1968 H3N2) for T cell assays
add to cart
PM-INFA-MP1-H1N1
PepMix™ Influenza A (MP1 /California (H1N1))
Pool of 61 peptides derived from a peptide scan through Matrix protein 1 of Influenza A virus (A/California/08/2009(H1N1)) for T cell assays
add to cart
PM-INFA_MP2
PepMix™ Influenza A (MP2 /California (H1N1))
Pool of 22 peptides derived from a peptide scan through Matrix protein 2 of Influenza A virus (A/California/08/2009(H1N1)) for T cell assays
add to cart
PM-INFA_NA
PepMix™ Influenza A (Neuroamidase /California (H1N1)
Pool of 115 peptides derived from a peptide scan through Neuraminidase of Influenza A virus (A/California/08/2009(H1N1)) for T cell assays
add to cart
PM-INFA_NP
PepMix™ Influenza A (NP (H3N2))
Pool of 122 peptides derived from a peptide scan through Nucleocapsid protein of Influenza A virus (strain A/Kitakyushu/159/1993 H3N2) for T cell assays
add to cart
RT-INFA-HA
RepliTope™ Influenza A (HA /Brisbane (H1N1))
Peptide microarray displaying 139 peptides derived from a peptide scan through Hemagglutinin of Influenza A virus (A/Brisbane/59/2007(H1N1))
add to cart
RT-INFA-HACal
RepliTope™ Influenza A (HA /California (H1N1))
Peptide microarray displaying 140 peptides derived from a peptide scan through Hemagglutinin of Influenza A virus (A/California/08/2009(H1N1))
add to cart
RT-INFA_MP1
RepliTope™ Influenza A (MP1 (H3N2))
Peptide microarray displaying 61 peptides derived from a peptide scan through Matrix protein 1 of Influenza A virus (strain A/Aichi/2/1968 (H3N2))
add to cart
RT-INFA-MP1-H1N1
RepliTope™ Influenza A (MP1 /California (H1N1))
Peptide microarray displaying 61 peptides derived from a peptide scan through Matrix protein 1 of Influenza A virus (A/California/08/2009(H1N1))
add to cart
RT-INFA_MP2
RepliTope™ Influenza A (MP2 /California (H1N1))
Peptide microarray displaying 22 peptides derived from a peptide scan through Matrix protein 2 of Influenza A virus (A/California/08/2009(H1N1))
add to cart
RT-INFA_NA
RepliTope™ Influenza A (Neuroamidase /California (H1N1)
Peptide microarray displaying 115 peptides derived from a peptide scan through Neuraminidase of Influenza A virus (A/California/08/2009(H1N1))
add to cart

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Quality Assurance

All production is performed according to ISO 9001:2015 standards.

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Contact us:

JPT Peptide Technologies GmbH
Volmerstrasse 5
12489 Berlin, Germany
Technical Support:
T: +49-30-6392-7878
X: +49-30-6392-7888
e-mail: peptide@jpt.com
USA & Canada:
T: 1.888.JPT.COM0 (1.888.578.2660)
X: 1.888.JPT.COM6 (1.888.578.2666)
e-mail: peptide@jpt.com