PepMix™ Mouse (WT1)

Product Code: PM-MM-WT1

Pool of 110 peptides derived from a peptide scan (15mers with 11 aa overlap) through Wilms tumor protein homolog (Swiss-Prot ID: P22561) of Mus musculus (Mouse) for T cell assays (e.g. ELISPOT).
Amount: 1 vial containing 15 nmol (appr. 25µg) per peptide
Purity: >70% (HPLC-MS)
Delivery Format: Freeze dried in amber type glass vial
Application(s): T-cell assays, Immune monitoring, Antigen specific T-cell stimulation, T-cell expansion, Cellular immune response
Indication(s)/Topic(s): Cancer, Wilms tumor 1
Delivery Time: 2-5 days

PepMixes™ Peptide Pools
The use of complete protein-spanning mixtures of overlapping peptides (PepMix™) is extremely efficient for immunostimulation of T-lymphocytes and diagnostic applications. In order to establish reliable and validated T-cell assays such as ELISPOT, appropriate positive and negative controls are essential. Selection of such controls helps to confirm proper functionality of the assay as well as viability of the cells used. In addition, high quality controls are most important to establish standardized assay protocols in clinical setups. Compared to commonly used controls like phytohemagglutinin (PHA) and concanavalin A (ConA) that stimulate T-cells as well as other cell types or full length antigens, synthetic peptide pools offer the advantage of a high batch-to-batch reproducibility, applicability of reliable chemical and biochemical QC/QA measures and shelf stability when stored as freeze dried aliquots.

Benefits of PepMix™
- Equivalent or better stimulation of CD4⁺ and CD8⁺ T-cell responses compared to whole protein antigens
- Simultaneous detection of CD4⁺ and CD8⁺ responses in a single sample
- Improved responses in stored blood and PBM cells compared to whole protein antigens
- More reliable as control than phytohemagglutinin (PHA) and concanavalin A (ConA)
- High-batch-to-batch reproducibility
- Reliable chemical and biochemical quality control and quality assurance for peptide synthesis and peptide pool generation
- Prolonged shelf stability when stored freeze dried

PepMix™ Mouse (WT1)

References:
Read References with PepMix™ Peptide Pools - Tumor Associated Antigens

Application Notes
"Developing Multi-HIV Antigen Specific T-Cells as a Component of a Cure Strategy"
Lam et al. (2015) Full text
"Peptide-stimulated Expansion of Virus-specific T cells For Preventative Treatment After Allogeneic Stem Cell Transplantation"
Gary et al. (2015) Full text