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Annotated PhosphoSites-Tyr-Phosphatase

Annotated PhosphoSites-Tyr-Phosphatase
  
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Delivery in 10-12 days
191,00 €
191,00 €

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Annotated PhosphoSites-Tyr-Phosphatase

Product Code: PHOS-MA-PY

Collection of >6000 phosphorylation sites as phosphotyrosine containing peptides displayed on one peptide microarray.
Amount: 2 peptide microarrays (each peptide is deposited 1xper subarray, 3 subarrays per slide), one for target incubation and one for control incubation + dummy slides and spacers (needed for manual incubation)
Delivery Format: Peptides immobilized onto glass slide (3 x 1 inches; 75 by 25 mm)
Application(s): Phosphosite detection, Substrate identification
Indication(s)/Topic(s): Phosphatase
Delivery Time: 10-12 days

Phosphatase Substrate Microarray
JPT Peptide Technologies applies its unique peptide microarray platform to generate peptide microarrays on glass slides for characterization of enzyme target families such as phosphatases. Phosphorylated peptides are immobilized on glass slides via a flexible linker. Chemoselective coupling generates microarrays displaying covalently attached peptides. Multiple identical copies of each microarray are produced. Incubation can be performed manually or automated. Read-out via fluorescence results in low background and high sensitivity.

Benefits of JPT's Phosphatase Substrate Microarray
- Get hundreds of identical microarray copies
- Peptides are free of truncated sequences
- Small amounts of your precious samples are needed for incubation
- Applicable to a variety of biological samples (e.g. serum, lysate, phosphatase)
- Low background on glass surface. Read-out via fluorescence
- Save time for HTS assay setup
- Screen thousands of potential peptide substrates economically

Annotated PhosphoSites-Tyr-Phosphatase

References:
Read References with Enzyme Substrate Microarrays

Application Notes for Enzyme Substrate Microarrays
"Exploring the human acetylome using high-density peptide microarrays"
Masch et al., Application Note (2012) (full text)
"Unraveling Phosphorylation of the Splicing Factor SC35 by the Dual Signaling Kinase PKC in Human T-Cells"
Rao et al., Application Note (2016) (full text)
"Use of High-Density Histone Peptide Arrays for Parsing the Specificity of a Histone Modifying Enzyme Complex"
Wilczek et al., Application Note (2013) (full text)

Testimonial
"We have successfully applied JPT's peptide microarrays as a screening tool for potential kinases transfering phosphate to phosphorylation sites identified by mass spectrometry. The triplicate alignment as well as the thorough selection of controls enabled generation of high quality data confirming our in vivo data generated by means of affinity chromatographic procedures."
Marcelo P. Coba, PhD, The Wellcome Trust Sanger Institute, Cambridge, UK

Annotated PhosphoSites-Tyr-Phosphatase

 
Code
Product Name
 
Price
Buy
PhSS-360-250
Phosphatase Substrate Set
Collection of 360 phosphopeptides derived from phosphorylation sites of Homo sapiens (Human), Mus musculus (mouse) and others
add to cart
PhSS-Y-360-250
Tyrosine-Phosphatase Substrate Set
Collection of 360 phosphotyrosine containing peptides derived from human phosphorylation sites of Homo sapiens (Human), Mus musculus (mouse) and others
add to cart

Description

Product Code: PHOS-MA-PY

Collection of >6000 phosphorylation sites as phosphotyrosine containing peptides displayed on one peptide microarray.
Amount: 2 peptide microarrays (each peptide is deposited 1xper subarray, 3 subarrays per slide), one for target incubation and one for control incubation + dummy slides and spacers (needed for manual incubation)
Delivery Format: Peptides immobilized onto glass slide (3 x 1 inches; 75 by 25 mm)
Application(s): Phosphosite detection, Substrate identification
Indication(s)/Topic(s): Phosphatase
Delivery Time: 10-12 days

Phosphatase Substrate Microarray
JPT Peptide Technologies applies its unique peptide microarray platform to generate peptide microarrays on glass slides for characterization of enzyme target families such as phosphatases. Phosphorylated peptides are immobilized on glass slides via a flexible linker. Chemoselective coupling generates microarrays displaying covalently attached peptides. Multiple identical copies of each microarray are produced. Incubation can be performed manually or automated. Read-out via fluorescence results in low background and high sensitivity.

Benefits of JPT's Phosphatase Substrate Microarray
- Get hundreds of identical microarray copies
- Peptides are free of truncated sequences
- Small amounts of your precious samples are needed for incubation
- Applicable to a variety of biological samples (e.g. serum, lysate, phosphatase)
- Low background on glass surface. Read-out via fluorescence
- Save time for HTS assay setup
- Screen thousands of potential peptide substrates economically

References

References:
Read References with Enzyme Substrate Microarrays

Application Notes for Enzyme Substrate Microarrays
"Exploring the human acetylome using high-density peptide microarrays"
Masch et al., Application Note (2012) (full text)
"Unraveling Phosphorylation of the Splicing Factor SC35 by the Dual Signaling Kinase PKC in Human T-Cells"
Rao et al., Application Note (2016) (full text)
"Use of High-Density Histone Peptide Arrays for Parsing the Specificity of a Histone Modifying Enzyme Complex"
Wilczek et al., Application Note (2013) (full text)

Testimonial
"We have successfully applied JPT's peptide microarrays as a screening tool for potential kinases transfering phosphate to phosphorylation sites identified by mass spectrometry. The triplicate alignment as well as the thorough selection of controls enabled generation of high quality data confirming our in vivo data generated by means of affinity chromatographic procedures."
Marcelo P. Coba, PhD, The Wellcome Trust Sanger Institute, Cambridge, UK

Documentation

Properties

Related Products

PhSS-360-250
Phosphatase Substrate Set
Collection of 360 phosphopeptides derived from phosphorylation sites of Homo sapiens (Human), Mus musculus (mouse) and others
add to cart
PhSS-Y-360-250
Tyrosine-Phosphatase Substrate Set
Collection of 360 phosphotyrosine containing peptides derived from human phosphorylation sites of Homo sapiens (Human), Mus musculus (mouse) and others
add to cart

Quality Assurance

All production is performed according to ISO 9001:2015 standards.

ISO 9001

 
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Contact us:

JPT Peptide Technologies GmbH
Volmerstrasse 5
12489 Berlin, Germany
Technical Support:
T: +49-30-6392-7878
X: +49-30-6392-7888
e-mail: peptide@jpt.com
USA & Canada:
T: 1.888.JPT.COM0 (1.888.578.2660)
X: 1.888.JPT.COM6 (1.888.578.2666)
e-mail: peptide@jpt.com