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SpikeTides™ Set Histone heavy quantified - 1000 pmol

SpikeTides™ Set Histone heavy quantified - 1000 pmol
  
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4.113,00 €
4.113,00 €

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SpikeTides™ Set Histone heavy quantified - 1000 pmol

Product Code: SPT-His-TQL-hum-1000pm

Collection of 37 stable isotope-labeled and absolutely quantified proteotypic peptides for Histone H3 for direct usage in mass-spectrometry based proteomics (MRM). The set comprises wild-type peptides and their Lys(Ac), Lys(Me), Lys(Me2) and Lys(Me3) modifications.
Amount: Micronic rack with 37 individual peptides (1000 pmol per peptide)
Delivery Format: Micronic tube rack 
Application(s): Cancer biomarker research, MRM assays, Proteomics, SRM assays, Mass spectrometry
Indication(s)/Topic(s): Acetyltransferase, Cancer, Cell signaling, Epigenetics, Gene regulation & expression, Histone Deacetylase, Histones, Kinase, Methyltransferase, Nucleosome, Phosphatase
Delivery Time: 3 weeks

JPT's heavy labeled and quantified SpikeTides™ Sets
JPT produced a set of stable isotope labeled proteotypic peptides for post-translationally modified histone H3 peptides. The peptides of this set are absolutely quantified for usage as internal standards in mass spectrometry assays.

Benefits of JPT's heavy labeled & quantified SpikeTides™ Sets
- Absolute quantification of multiple proteins from a single sample
- First economic absolutely quantified peptide set available
- Multiplexed analysis of disease status and therapeutic success

SpikeTides™ Set Histone heavy quantified - 1000 pmol

References:
Read References with Histone Code Tools

Application Note
"Absolute Quantification of Metabolic Enzymes via Targeted Proteomics using a new Kit of SpikeTides™ Peptides"
Bindel et al., Application Note (2015) Full text

Testimonials
"My unit at the Banting and Best Department of Medical Research, University of Toronto Centre for Cellular and Biomolecular Research (CCBR) works on the systematic identification and quantification of proteins and protein complexes on a global level. In addition we have started to perform targeted proteomics to study protein regulation within pathways, e.g. signaling pathways during stem cell fate decision. For this we used JPT's various SpikeTides™ peptide products to build LC-MRM assays in a fast and cost efficient manner. More recently we also found new ways of using low cost SpikeTides™_TQL as quantitative peptide standards in order to quantify protein targets more accurately. We are currently in the process of validating this technology for the accurate quantification of various predicted microRNA targets."
Prof. Andrew Emili, Donnelly Centre for Cellular and Biomolecular Research, University of Toronto

"My group studies the proteomic composition of distinct chromatin domains, the mechanisms that operate to maintain the composition of histone modifications and the associated proteins. For precise and accurate identification and quantification of histone peptides that carry multiple post-translational modifications directly from biological samples JPT's SpikeTides™ _TQL peptide standards proved to be of excellent value for our research in various projects."
Prof. Dr. Axel Imhof, Adolf-Butenandt Institute, University of Munich, Germany

Description

Product Code: SPT-His-TQL-hum-1000pm

Collection of 37 stable isotope-labeled and absolutely quantified proteotypic peptides for Histone H3 for direct usage in mass-spectrometry based proteomics (MRM). The set comprises wild-type peptides and their Lys(Ac), Lys(Me), Lys(Me2) and Lys(Me3) modifications.
Amount: Micronic rack with 37 individual peptides (1000 pmol per peptide)
Delivery Format: Micronic tube rack 
Application(s): Cancer biomarker research, MRM assays, Proteomics, SRM assays, Mass spectrometry
Indication(s)/Topic(s): Acetyltransferase, Cancer, Cell signaling, Epigenetics, Gene regulation & expression, Histone Deacetylase, Histones, Kinase, Methyltransferase, Nucleosome, Phosphatase
Delivery Time: 3 weeks

JPT's heavy labeled and quantified SpikeTides™ Sets
JPT produced a set of stable isotope labeled proteotypic peptides for post-translationally modified histone H3 peptides. The peptides of this set are absolutely quantified for usage as internal standards in mass spectrometry assays.

Benefits of JPT's heavy labeled & quantified SpikeTides™ Sets
- Absolute quantification of multiple proteins from a single sample
- First economic absolutely quantified peptide set available
- Multiplexed analysis of disease status and therapeutic success

References

References:
Read References with Histone Code Tools

Application Note
"Absolute Quantification of Metabolic Enzymes via Targeted Proteomics using a new Kit of SpikeTides™ Peptides"
Bindel et al., Application Note (2015) Full text

Testimonials
"My unit at the Banting and Best Department of Medical Research, University of Toronto Centre for Cellular and Biomolecular Research (CCBR) works on the systematic identification and quantification of proteins and protein complexes on a global level. In addition we have started to perform targeted proteomics to study protein regulation within pathways, e.g. signaling pathways during stem cell fate decision. For this we used JPT's various SpikeTides™ peptide products to build LC-MRM assays in a fast and cost efficient manner. More recently we also found new ways of using low cost SpikeTides™_TQL as quantitative peptide standards in order to quantify protein targets more accurately. We are currently in the process of validating this technology for the accurate quantification of various predicted microRNA targets."
Prof. Andrew Emili, Donnelly Centre for Cellular and Biomolecular Research, University of Toronto

"My group studies the proteomic composition of distinct chromatin domains, the mechanisms that operate to maintain the composition of histone modifications and the associated proteins. For precise and accurate identification and quantification of histone peptides that carry multiple post-translational modifications directly from biological samples JPT's SpikeTides™ _TQL peptide standards proved to be of excellent value for our research in various projects."
Prof. Dr. Axel Imhof, Adolf-Butenandt Institute, University of Munich, Germany

Documentation

Properties

News

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SARS-CoV-2 SpikeMix  for Mass Spectrometry covers spike, NCAP, VME-1 & ORF9B
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> Read more news!

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All production is performed according to ISO 9001:2015 standards.

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JPT Peptide Technologies GmbH
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Technical Support:
T: +49-30-6392-7878
X: +49-30-6392-7888
e-mail: peptide@jpt.com
USA & Canada:
T: 1.888.JPT.COM0 (1.888.578.2660)
X: 1.888.JPT.COM6 (1.888.578.2666)
e-mail: peptide@jpt.com