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SpikeMix™ Kinase Activation Loops (Human) - heavy - 100pm

SpikeMix™ Kinase Activation Loops (Human) - heavy - 100pm
  
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2.188,00 €
2.188,00 €

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SpikeMix™ Kinase Activation Loops (Human) - heavy - 100pm

Product Code: SPT-KAL-POOL-L-100pm

Two pool of representative stable isotope labeled proteotypic peptides for human kinase activation loops for direct usage in mass-spectrometry based proteomics (MRM). One pool contains 178 non-phosphorylated, the other pool 288 phosphorylated peptides.
Amount: 288 phospho- and 178 non-phospho peptides (approx. 100pm per peptide) as 2 peptide mixes.
Delivery Format: Poly-propylene vial
Application(s):  SRM assays, MRM assays, Proteomics, Cancer biomarker research, Mass spectometry, Enzyme kinetic, Substrate identification, Substrate specificity
Indication(s)/Topic(s): Gene regulation, expression, Cancer, Breast cancer, Burkitt's lymphoma, Gastric cancer, Testis/ovary cancer, Cancer chemotherapy, Melanoma, Prostate cancer
Delivery Time: 2-5 days 

JPT's SpikeMix™ Kinase Activation Loops (Human) - heavy
JPT produced 466 representative stable isotope labeled proteotypic peptides (178 non-phosphorylated, 288 phosphorylated) for human kinase activation loops for direct usage in mass-spectometry based proteomics. The peptides are available as set of individual peptides are as peptide mixes (pools) with stable isotope labeled (using heavy Arg U-13C6, U-15N4 or Lys U-13C6, U-15N2) peptides. We suggest to setup experimental conditions with the SpikeTides™ Set before using the SpikeMix™ peptide pools, due to complex detection of phosphopeptides.

Benefits of JPT's SpikeMix™ Kinase Activation Loops (Human) - heavy
- Quantification of multiple proteins from a single sample
- First economic heavy peptide pool available
- Multiplexed analysis of disease status and therapeutic success
- Presence of each peptide confirmed (by LC-MS or Maldi)
- Custom absolutely quantified peptides available upon request

 

SpikeMix™ Kinase Activation Loops (Human) - heavy - 100pm

References:
Read References with Reference Peptides for Targeted Proteomics - SpikeTides™ & SpikeMix™

Application Notes
"Development of a Multiplexed Targeted SRM Assay for NCI’s Top Tumor Associated Antigens"
Soderblom et al., Application Note (2015) Full text
"Absolute Quantification of Metabolic Enzymes via Targeted Proteomics using a new Kit of SpikeTides™ Peptides"
Bindel et al., Application Note (2015) Full text
"Development and Characterization of SpikeMix™ ABRF (cross-Species Standard) Consisting of 1,000 Stable Isotope Labeled Peptides"
Colangelo, Application Note (2014) Full text

Testimonials
"As Director of the Protein Profiling at Yale Keck Biotechnology Resource Facility, I coordinated a collaboration between the Association of Biomolecular Resource Facilities (ABRF) standard proteomic research group (sPRG) and JPT Peptide Technologies to develop the ABRF cross-species SpikeMix™ peptide pool. The joint development turned out to be an extraordinary effective endeavor and the resulting product was qualified in more than 52 proteomics labs around the globe. I was impressed by JPT's scientific and technological capabilities as well as their enthusiasm to drive the project forward."
Christopher M. Colangelo, Director of Protein Profiling at Keck Biotechnology Resource Facility, Yale University, USA

"Our research at Stanford University seeks to discover unconventional translation products, and proteins made by unsequenced organisms; to characterize a wide array of protein post-translational modifications; and to perform peptide antigen binding assays. On all of these fronts, we are in need of high quality peptide standards for assay development and protein quantification. The successful collaboration with the team at JPT Peptide Technologies and their wide range of products has been a critical factor for advancing my group's research."
Joshua E. Elias, Ph.D., Assistant Professor, Chemical & Systems Biology, Stanford University School of Medicine, Clark Center, Stanford, CA

Description

Product Code: SPT-KAL-POOL-L-100pm

Two pool of representative stable isotope labeled proteotypic peptides for human kinase activation loops for direct usage in mass-spectrometry based proteomics (MRM). One pool contains 178 non-phosphorylated, the other pool 288 phosphorylated peptides.
Amount: 288 phospho- and 178 non-phospho peptides (approx. 100pm per peptide) as 2 peptide mixes.
Delivery Format: Poly-propylene vial
Application(s):  SRM assays, MRM assays, Proteomics, Cancer biomarker research, Mass spectometry, Enzyme kinetic, Substrate identification, Substrate specificity
Indication(s)/Topic(s): Gene regulation, expression, Cancer, Breast cancer, Burkitt's lymphoma, Gastric cancer, Testis/ovary cancer, Cancer chemotherapy, Melanoma, Prostate cancer
Delivery Time: 2-5 days 

JPT's SpikeMix™ Kinase Activation Loops (Human) - heavy
JPT produced 466 representative stable isotope labeled proteotypic peptides (178 non-phosphorylated, 288 phosphorylated) for human kinase activation loops for direct usage in mass-spectometry based proteomics. The peptides are available as set of individual peptides are as peptide mixes (pools) with stable isotope labeled (using heavy Arg U-13C6, U-15N4 or Lys U-13C6, U-15N2) peptides. We suggest to setup experimental conditions with the SpikeTides™ Set before using the SpikeMix™ peptide pools, due to complex detection of phosphopeptides.

Benefits of JPT's SpikeMix™ Kinase Activation Loops (Human) - heavy
- Quantification of multiple proteins from a single sample
- First economic heavy peptide pool available
- Multiplexed analysis of disease status and therapeutic success
- Presence of each peptide confirmed (by LC-MS or Maldi)
- Custom absolutely quantified peptides available upon request

 

References

References:
Read References with Reference Peptides for Targeted Proteomics - SpikeTides™ & SpikeMix™

Application Notes
"Development of a Multiplexed Targeted SRM Assay for NCI’s Top Tumor Associated Antigens"
Soderblom et al., Application Note (2015) Full text
"Absolute Quantification of Metabolic Enzymes via Targeted Proteomics using a new Kit of SpikeTides™ Peptides"
Bindel et al., Application Note (2015) Full text
"Development and Characterization of SpikeMix™ ABRF (cross-Species Standard) Consisting of 1,000 Stable Isotope Labeled Peptides"
Colangelo, Application Note (2014) Full text

Testimonials
"As Director of the Protein Profiling at Yale Keck Biotechnology Resource Facility, I coordinated a collaboration between the Association of Biomolecular Resource Facilities (ABRF) standard proteomic research group (sPRG) and JPT Peptide Technologies to develop the ABRF cross-species SpikeMix™ peptide pool. The joint development turned out to be an extraordinary effective endeavor and the resulting product was qualified in more than 52 proteomics labs around the globe. I was impressed by JPT's scientific and technological capabilities as well as their enthusiasm to drive the project forward."
Christopher M. Colangelo, Director of Protein Profiling at Keck Biotechnology Resource Facility, Yale University, USA

"Our research at Stanford University seeks to discover unconventional translation products, and proteins made by unsequenced organisms; to characterize a wide array of protein post-translational modifications; and to perform peptide antigen binding assays. On all of these fronts, we are in need of high quality peptide standards for assay development and protein quantification. The successful collaboration with the team at JPT Peptide Technologies and their wide range of products has been a critical factor for advancing my group's research."
Joshua E. Elias, Ph.D., Assistant Professor, Chemical & Systems Biology, Stanford University School of Medicine, Clark Center, Stanford, CA

Documentation

Properties

Quality Assurance

All production is performed according to ISO 9001:2015 standards.

ISO 9001

 
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JPT Peptide Technologies GmbH
Volmerstrasse 5
12489 Berlin, Germany
Technical Support:
T: +49-30-6392-7878
X: +49-30-6392-7888
e-mail: peptide@jpt.com
USA & Canada:
T: 1.888.JPT.COM0 (1.888.578.2660)
X: 1.888.JPT.COM6 (1.888.578.2666)
e-mail: peptide@jpt.com